What is the most specific liver test? AST, ALT, ALP, or albumin Aminotransferases AST and ALT are liver enzymes that catalyze transfer of amino groups in gluconeogenesis. AST catalyzes the transfer of an amino group from aspartic acid to ketoglutaric acid to produce oxaloacetic acid. ALT catalyzes the transfer of an amino group from alanine to ketoglutaric acid to produce pyruvic acid. AST is found in many tissues including the liver, skeletal muscle, cardiac muscle, kidney, brain, pancreas, lungs and red blood cells. ALT is found mainly in the liver and is more specific for liver disease than AST. Alkaline phosphatase ALP is found in the liver, but also in…